| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeVries, Clint A (14)
|
VACGBD |
27.29 |
26.83 |
-0.46 |
| 2 |
Ward, James F (14)
|
VACGBD |
27.55 |
27.20 |
-0.35 |
| 3 |
Garth, Ian A (14)
|
VATIDE |
27.11 |
27.52 |
0.41 |
| 4 |
Isely, Gabriel N (14)
|
VACGBD |
28.16 |
27.87 |
-0.29 |
| 5 |
Salpeter, Troy M (13)
|
VATIDE |
27.91 |
28.02 |
0.11 |
| 6 |
Bradley, Connor W (13)
|
VATIDE |
27.96 |
28.29 |
0.33 |
| 7 |
Kim, Bernard S (14)
|
VACGBD |
28.17 |
28.45 |
0.28 |
| 8 |
Brant, Tanner J (14)
|
VAODAC |
29.68 |
29.32 |
-0.36 |
| 9 |
Rodgers, Caden R (13)
|
VACGBD |
30.58 |
29.73 |
-0.85 |
| 10 |
Stowell, Daniel C (13)
|
VATIDE |
28.80 |
30.05 |
1.25 |
| 11 |
Stowell, Ethan H (14)
|
VATIDE |
30.58 |
30.35 |
-0.23 |
| 12 |
Ziemba, Peter L (14)
|
VACGBD |
30.98 |
30.64 |
-0.34 |
| 13 |
Krum, Braden K (14)
|
VAUN-T |
29.90 |
30.93 |
1.03 |
| 14 |
Delipetros, Apostolos M (13)
|
VAODAC |
32.88 |
31.94 |
-0.94 |
| 15 |
Sievenpiper, Josef E (13)
|
VACGBD |
31.17 |
31.98 |
0.81 |
| 16 |
Garth, Logan D (13)
|
VATIDE |
33.51 |
32.64 |
-0.87 |